NEW – SpectraMax® M2Multimode Microplate Readers, with SoftMax® Pro Software, Molecular Devices

SpectraMax M2/M2e Microplate Readers Multi-detection microplate readers with dual-mode cuvette ports

Introduction
The SpectraMax® M2 and M2e Systems
from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports,
and top- and bottom-reading capability
(top-reading only on the M2). Detection
modalities include absorbance (UV-Vis Abs)
and fluorescence intensity (FI). Endpoint,
kinetic, spectrum and area-well scanning
read types and the PathCheck® Sensor
allow homogeneous and heterogeneous
microplate assays to be performed in
one flexible system. SpectraMax M2
readers provide for easy conversion and
optimization of very-low-throughput to
medium-high-throughput assays, faster,
more precise results and reagent savings.
In addition, combined absorbance and
fluorescence intensity assays can be run by
issuing a single read command.

Dual monochromators
With SpectraMax M2/M2e readers, there is
no need for expensive filters. The system
uses two scanning monochromators to
determine optimal excitation and emission
settings. Changing methods or fluorophores
requires only a few mouse selections to
optimize the system.
Patented PathCheck sensor
The PathCheck sensor from Molecular
Devices is the only patented†
technology
available that measures the depth (optical
pathlength) of samples in a microplate. With
SoftMax® Pro Software, it can automatically
normalize the well absorbance to a cuvette
equivalent pathlength of 1 cm—similar to
using 96 or 384 cuvettes simultaneously.
The PathCheck sensor allows standard
curves to be eliminated, and for compounds
with a known extinction coefficient,
concentration can be calculated directly
from absorbance.

Applications
• DNA/RNA/protein quantitation and purity
• PicoGreen/NanoOrange/Bradford
• ELISAs/enzyme kinetics (i.e., Km, Ki, etc.)
• Drug dissolution profiles
• Live/Dead Viability/cytotoxicity assays
• Caspase-3 and protease assays
• cAMP assays using CatchPoint® Assay Kits
• Low volume applications
Comprehensive data analysis
SoftMax Pro Software provides data acquisition, analysis and
management capabilities, allowing cross-plate analysis and
custom calculations. There is no need to export data to a
spreadsheet program.
Instrument and software validation
The SpectraTest® ABS1 Absorbance and SpectraTest FL1
Fluorescence Validation Test Plates provide validation of the
system’s optical characteristics. The SoftMax Pro Software
validation package and IQ/OQ validation protocols include tools for
FDA 21 CFR Part 11 compliance.
Plate stacker and robot integration
The SpectraMax M2 and M2e readers can be integrated with the
StakMax® Microplate Stacker from Molecular Devices in a matter
of minutes. For a higher degree of automation, the Automation
Vendor Partners Program has streamlined the integration of our
microplate reader systems with all leading partner robots.
Highly customizable low volume applications
Molecular Devices’ unique SpectraDrop™ Micro-Volume
Microplate offers the highest throughput solution for low volume
measurement available on the market today. Innovative and
flexible design features enable accelerated sample preparation
time and increased laboratory productivity in DNA, RNA and
protein research. The SpectraDrop microplate assures uniform and
reproducible analysis and integrates seamlessly with the StakMax
stacker for greater research capacity.