The Opentrons® OT-2 is a high-precision, open-source liquid handler designed to be easy for scientists to use and flexible enough to automate many application workflows. This robot and its accompanying automation platform are used to automate hundreds of protocols and workflows in genomics, proteomics, cell-based assays, and drug discovery.
The OT-2 has a user-friendly, plug and play graphical interface to design protocols compatible with any ANSI/SBS compliant or automation friendly labware. The base model includes an Opentrons GEN2 Single-Channel Pipette; you can also use up to two pipettes at the same time. Additional pipettes can be purchased separately and are easily interchangeable by the user. Consumables and other labware are sold separately.
Weight
48 kg or 105.8 lb
Pipette configurations
Single- and 8-channel pipetting
2 pipette mounts, for a configuration of 1 or 2 single- or 8-channel pipettes. Pipettes are easily interchangeable.
Pipette volumes and specs
Single-channel: 1-1000 µL
8-channel: 1-300 µL
Opentrons Pipette Whitepaper
Single-Channel Manual
8-channel manual
Deck
11 deck slots enable countless configurations
Deck slots are compatible with standard SBS dimensions.
Deck also includes a removable trash bin.
Connectivity
WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0
Learn More
Video
Integrated video camera (Included for future or custom functionality)
Audio
Integrated audio system (Included for future or custom functionality)
Power requirements
Robot Power Input: 36 VDC, 6.1 A
Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC
For additional power requirement information, please review this support article
Frame composition
Rigid steel and CNC aluminum design
Operating temperature & relative humidity
Recommended 20-24 °C
Recommended 40-60% RH
Learn more
Time to fill 96-well plate
Pipette Distribute,
one tip Transfer,
same tip Transfer,
new tip
P20 8-Channel 0:22 1:35 4:19
P300 8-Channel 0:26 1:38 4:24
P20 Single-Channel 3:12 13:10 36:01
P300 Single-Channel 4:01 13:39 41:31
P1000 5:54 15:21 44:50
Single-channel accuracy & precision
Pipette Volume Random Error Systematic Error
%D µl %CV µl
P20 1
10
20 ±15%
±2%
±1.5% 0.15µl
0.2µl
0.3µl ±5%
±1%
±0.8% 0.05µl
0.1µl
0.16µl
P300 20
150
300 ±4%
±1%
±0.6% 0.8µl
1.5µl
1.8µl ±2.5%
±0.4%
±0.3% 0.05µl
0.6µl
0.9µl
P1000 100
500
1000 ±2%
±1%
±0.7% 2.0µl
5.0µl
7.0µl ±1%
±0.2%
±0.15% 1µl
1µl
1.5µl
8-channel accuracy & precision
Pipette Volume Random Error Systematic Error
%D µl %CV µl
P20 1
10
20 ±20%
±3%
±2.2% 0.2µl
0.3µl
0.44µl ±10%
±2%
±1.5% 0.1µl
0.2µl
0.3µl
P300 20
150
300 ±10%
±2.5%
±1.5% 2.0µl
3.75µl
4.5µl ±4%
±0.8%
±0.5% 0.8µl
1.2µl
1.5µl
Sterility
Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment
Add the Opentrons HEPA Module, or add your own UV lights
Viscosity
For best practices on automating viscous liquid handling, review our application note
Viscous Liquid Handling Automation using Opentrons OT-2 ›
API compatibility
Interfaces with any product that has an API
Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.
Learn more
Interface
Windows 10, macOS 10.10 or later, Ubuntu 12.04 or later.
Opentrons App
Tip compatibility
Use Opentrons Tips and Opentrons Filter Tips
Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.
Labware compatibility
Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.
Learn more
OT-2 Manual
Warranty
One year warranty against manufacturer defects
Stress tested to last a minimum of 3 years of full-time use
Learn more
Return policy
60 day, 100% risk free
Learn more
Certifications
Certifications complete (CE, FCC, NRTL, CB, ISO 9001)
Not certified/validated (IVD, GMP)
Example Commands
Tip Handling
Pick up tip
Drop tip
Return tip
New tip (always, never, once)
More…
Liquid Control
Aspirate
Dispense
Aspirate speed
Dispense speed
Blowout
Touch tip
Mix
Air gap
Utility
Move to
Delay
User-specified pause
Homing
Comment
Transfer
Basic transfer
Volumes > Pipette Volume
Distribute
Consolidate
Variable Settings
Speed
Volume
Count
Other
Dispose
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