Prevent costly delays with an enhanced understanding of sample quality using the Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometers. Innovative Thermo Scientific™ Acclaro™ Sample Intelligence technology is built into every instrument for improved measurement accuracy and contaminant identification. Quantify and qualify DNA, RNA, and protein samples in seconds with only 1-2 μL, and obtain full-spectral data before you decide to use samples in downstream applications. Additional cuvette position also available.
Move one step closer to your research goals with an ergonomic, standalone spectrophotometer with high-resolution, touch screen interface. Powerful auto-range pathlength technology facilitates accurate measurements for concentrated samples with no need for dilutions. This is your innovative alternative to the NanoDrop 2000/2000c Spectrophotometers.
Acclaro Sample Intelligence Technology
Acclaro technology enhances user understanding of sample quality while delivering accurate quantitative measurements. Early identification of contaminants can prevent failure of downstream applications and save days of troubleshooting.
- Verify sample quality in seconds – identify sample contaminants and obtain corrected concentration results.
- Access information through alerts – receive instant feedback about sample quality with on-demand technical support and guided troubleshooting.
- Rely on accurate results – ensure measurement integrity with embedded sensor and digital image analysis.
- Identify nucleic acid contaminants such as RNA contamination in dsDNA samples and chemical contaminants such as phenol in RNA samples.
- Wide spectral range (190-850 nm) for measuring a variety of samples types:
- Peptides (205 nm)
- DNA and RNA (260 nm)
- Purified protein (280 nm)
- Toxicology assays and industrial dyes (490 nm)
- Gold nanoparticles (520 nm)
- Colorimetric protein assays (BCA 562 nm, Bradford 595 nm, Modified Lowry 650 nm, Pierce 660 660 nm)
- Optical Density measurements (600 nm)
- Combining the patented** sample-retention system with cuvette capability accommodates low and high concentrations (2.0 – 27,500 ng/μL dsDNA and 0.06 – 820 mg/mL BSA)
- Pedestal measurements that require only 1 – 2 μL of your sample and do not require sample dilution, even for highly concentrated samples
- Calculates sample purity ratios (A260/A280 nm and A260/A230 nm)
- Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry
- User-friendly software includes custom methods and data export capabilities
NanoDrop One and Oneᶜ Spectrophotometer specifications
Category | Attribute | Description |
Samples | Minimum Sample Size | 1 μL |
Sample Number | 1 | |
Limit of Detection | Pedestal: 2 ng/μL (dsDNA), 0.06 mg/mL (BSA)
Cuvette (NanoDrop OneC only): 0.2 ng/μL (dsDNA), 0.006 mg/mL (BSA) |
|
Maximum Concentration | Pedestal: 27,500 ng/μL (dsDNA), 820 mg/mL (BSA) | |
Cuvette (NanoDrop OneC only): 75 ng/μL (dsDNA) | ||
Optics | Pathlength(s) | 0.030 to 1.0 mm automatically adjusting |
Light Source | Xenon flash lamp | |
Detector Type | 2048-element CMOS linear image sensor | |
Wavelength Range | 190–850 nm | |
Wavelength Accuracy | ±1 nm | |
Spectral Resolution | ≤1.8 nm (FWHM at Hg 254 nm) | |
Typical Measurement Repeatability | 0.002 A (1.0 mm path) or 1% CV, whichever is greater | |
Absorbance Accuracy1 | 3% (at 0.97 A, 302 nm) | |
Absorbance Range (10 mm equivalent) | Pedestal: 0–550A
Cuvette (NanoDrop OneC only): 0–1.5A |
|
Measurement and Data Processing Time | 8 seconds | |
21 CFR Part 11 Compliance | Yes, optional | |
Characteristics | Instrument Control | Built-in touchscreen or PC Control with free PC software |
Footprint (W × D × H) | 20 × 25.4 × 32.3 cm (8 × 10 × 12.7 in.) | |
Weight | 3.6 kg | |
Sample Pedestal | 303 stainless steel and quartz fiber | |
Cuvette Position | Optional (with stirring) (NanoDrop OneC only) | |
Operating Voltage | 12 V (DC) | |
Operating Power Consumption | 12–18 W | |
System | Instrument Control | Built-in touchscreen |
Applications Support | Nucleic Acid A260, A260/A280, A260/A230, Labeled Nucleic Acids, Microarray, dsDNA, ssDNA, RNA, Oligo DNA/Oligo RNA, RNA/DNA Deconvolution, Custom Factor; Protein A280 and A205, Protein Pierce 660, Protein Bradford, Protein BCA, Protein Lowry, Proteins & Labels. Protein Editor; Dye Editor, OD600, Kinetics, UV-Vis, and Custom Methods | |
Language Support | Chinese, French, German, Japanese, Korean, Polish, Spanish, English | |
Accessory Support | DYMO® LabelWriter® 550 Turbo printer, Bluetooth keyboard, mouse, and barcode reader | |
Software Compatibility | Standalone, Local Control software
NanoDrop One PC Control (free download) Thermo Scientific Security Suite (purchase separately) |
|
Connectivity | Three USB-A ports, Ethernet, Bluetooth, and Wi-Fi* | |
Advanced Connectivity | NanoDrop One Cloud Application2
Thermo Fisher Connect Platform |
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