The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs.
Features and Benefits
- All-in-one flexible imaging — get precise, reproducible fluorescence, chemiluminescence, and colorimetric gel and blot detection, analysis and documentation in a single system
- Improve your imaging capabilities with the ChemiDoc MP — benefit from the advantages of using a wider range of fluorescent reporters (RGB, far red, and near infrared) including StarBright Fluorophores; see the fluorophore compatibility chart
- Multiplex fluorescent western blotting — detect up to three proteins simultaneously and eliminate the need for stripping and reprobing
- Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
- Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
- Sample Trays and Smart Tray Technology — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
- Installation/Operational Qualification kit available for ensuring installation and calibration is properly executed for highly regulated laboratory environments.
Performing Stain-Free western blotting using the ChemiDoc MP System provides the ability to rapidly assess electrophoresis and blot transfer quality prior to blot processing, to perform total protein normalization and multiplexing using a wide rance of fluorophores.
Applications and Uses
- Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
- Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with fluorescence, chemiluminescence, or colorimetric detection
Specifications
Smart Tray Technology™
Automatic recognition of application-specific tray and adjustment of imaging parameters and software options
Autofocus
Precalibrated focus for any zoom setting or sample height
Auto-exposure
Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications
Image flat fielding
Dynamic; precalibrated and optimized for each application
Hardware
Touch-screen functionality
Multitouch capability (2 points) 12.1″ display
Maximum image area (W x H)
21 x 16.8 cm
Detector
Cooled CCD, 6 megapixels
Dynamic range
>4 orders of magnitude
Illumination sources
Trans-UV, 302 nm excitation
Epi-white
Trans-white (requires White Sample Tray)
Trans-blue, 450−490 nm excitation (requires Blue Sample Tray)
Epi-blue, 460−490 nm excitation
Epi-green, 520−545 nm excitation
Epi-red, 625−650 nm excitation
Epi-far red, 650−675 nm excitation
Epi-near IR, 755−777 nm excitation
Epi-white
Trans-white (requires White Sample Tray)
Trans-blue, 450−490 nm excitation (requires Blue Sample Tray)
Epi-blue, 460−490 nm excitation
Epi-green, 520−545 nm excitation
Epi-red, 625−650 nm excitation
Epi-far red, 650−675 nm excitation
Epi-near IR, 755−777 nm excitation
Filter and application
590/110 nm standard filter to perform protein and DNA gel and blot imaging
Chemiluminescence filter
518–546 nm filter for blue-excitable fluorophores and stains
577–613 nm filter for green-excitable fluorophores and stains
675–725 nm for red-excitable fluorophores and stains
700–730 nm filter for far red-excitable fluorophores and stains
813–860 nm for near IR-excitable fluorophores and stains
Chemiluminescence filter
518–546 nm filter for blue-excitable fluorophores and stains
577–613 nm filter for green-excitable fluorophores and stains
675–725 nm for red-excitable fluorophores and stains
700–730 nm filter for far red-excitable fluorophores and stains
813–860 nm for near IR-excitable fluorophores and stains
Data output
16-bit or 8-bit: SCN, TIFF, JPEG files
Instrument weight
35 kg (78 lb)
Instrument size (L x W x H)
61 x 51 x 53 cm
Operating voltage
100–250 V
Operating temperature
10–28°C
Operating humidity
10–85% relative humidity (non-condensing)
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